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腾博官网高分文件:蚯蚓SOD,CAT,MDA ELISA试剂盒引用文件
【文件标题】Assessing the bioavailability and biotoxicity of spiromesifen and its main metabolite spiromesifen-enol (M01) reveals the defense mechanisms of earthworms (Eisenia fetida)
【作者】Jianwei Fang , Binning Wang , Kuan Fang ,et.al
【作者单元】中国农业科学院（ Chinese Academy of Agricultural Sciences）
【文件中引用产品】
蚯蚓超氧化物歧化酶（SOD）ELISA试剂盒活性
蚯蚓过氧化氢酶（CAT）ELISA试剂盒活性
蚯蚓丙二醛（MDA）ELISA试剂盒
【关键词】UPLC-MS/MS,Transcriptome sequencing,SEM,TEM,Common DEGs
【DOI】http://dx.doi.org/10.1016/j.scitotenv.2021.151910
【影响因子(IF)】10.75
【出版期刊】《Science of the Total Environment》
【产品原文引用】
2.6. Determination of oxidative and immune stress effects
The protein content was determined according to the manufacturer's instructions using a protein assay kit (Solarbio; China). The reaction between Coomassie brilliant blue and protein was monitored at 595 nm, and the protein content was determined based on the change in absorbance. The ·OH? content, SOD activity, CAT activity, and MDA content were measured using an ELISA kit (Shanghai Hengyuan Biotechnology Co., Ltd.; China). The contents or activities were determined according to the changes in the absorbance induced by the color reaction.
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