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HA-Tag Monoclonal An...

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HA-Tag Monoclonal Antibody

  腾博官网生物抗体平台现已占有4万多种抗体(含单抗和多抗),,, ,,蕴含人类常用抗体,,, ,,模式生物类有关抗体、幼分子抗体、二抗、标签抗体及内参抗体等。。。。。。。。并以每年4000种的速度在持续增长。。。。。。。。

  腾博官网生物占有专业的质控团队,,, ,,建设先进尝试仪器,,, ,,现已成功地成立了ChIP平台,,, ,,因而腾博官网抗体不仅能提供Biotin、FITC、HRP等象征,,, ,,大部门抗体都能进行ELISA、WB、IHC、IF、IP、ChIP蹬爪用验证。。。。。。。。

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HA-Tag Monoclonal Antibody

 
  • 货号:
    HYK-200061
  • 规格:
     
  • 图片:
    • Western Blot
      Positive WB detected in: 3 different overexpression lysates with HA tagged
      All lanes: HA-Tag antibody at 1:1000
      Secondary
      Goat polyclonal to Mouse IgG at 1/10000 dilution
      Predicted band size: 35, 35, 48 kDa
      Observed band size: 35, 35, 48 kDa

    • Western Blot
      Positive WB detected in: HA-tagged fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng, 1.5625ng
      All lanes: HA-Tag antibody at 1:1000
      Secondary
      Goat polyclonal to Mouse IgG at 1/10000 dilution
      Predicted band size: 35 kDa
      Observed band size: 35 kDa

    • Western Blot
      Positive WB detected in: HA-tagged fusion protein
      All lanes: HA-Tag antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000
      Secondary
      Goat polyclonal to Mouse IgG at 1/10000 dilution
      Predicted band size: 35 kDa
      Observed band size: 35 kDa

    • Western Blot
      Positive WB detected in: 6 different recombinant proteins with HA tagged, Hela whole cell lysate, 3T3 whole cell lysate
      All lanes: HA-Tag antibody at 1:5000
      Secondary
      Goat polyclonal to Mouse IgG at 1/10000 dilution

    • Immunofluorescence staining of 293F cells with CSB-MA000141M0m at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

产品详情

  • 别号:
    HA-Tag
  • 宿主:
    Mouse
  • 反映种属:
    All
  • 免疫原:
    YPYDVPDYA synthetic peptide conjugate to KLH
  • 象征方式:
    Non-conjugated
  • 克隆类型:
    Monoclonal
  • 抗体亚型:
    IgG2b
  • 纯化方式:
    >95%, Protein A purified
  • 克隆号:
    18B11H6
  • 浓度:
    It differs from different batches. Please contact us to confirm it.
  • 保留缓冲液:
    Preservative: 0.03% Proclin 300
    Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
  • 产品提供大局:
    Liquid
  • 利用领域:
    ELISA, WB, IF, IP, FC
  • 推荐稀释比:
    Application Recommended Dilution
    WB 1:5000-1:160000
    IF 1:50-1:200
    IP 1?g-5?g
    FC 1:50-1:200
  • Protocols:
    ELISA Protocol
    Western Blotting (WB) Protocol
    Immunofluorescence (IF) Protocol
    Immunoprecipitation (IP) Protocol
    Flow cytometry (FC) Protocol
  • 贮存前提:
    Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
  • 货期:
    Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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